Regulatory

Part:BBa_J119412:Design

Designed by: Isabella Wiebelt-Smith   Group: Eckdahl Lab   (2018-11-07)


IM1 - i-motif sequence on pTac template strand


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The loop sizes of the i-motif had to be adjusted. The optimal number of base pairs between the -35 and -10 regions is 17, so the size of the loops was limited and the i-motif sequence had to start upstream of the -35 region.


Source

It is a combination of pTac, and a modification of the Nuclease Hypersensitive Element III in the promoter of the human c-Myc gene.

References